DCs were pulsed with RM-1 prostate cancer cell lysate, and IL-12 was assay in the supernatant of sensitized DC culture fluid by enzyme linked immunosorbent assay ( ELISA). The effect of inducing specific kill activity of DC vaccine was detected. RM-1前列腺癌细胞裂解物产物致敏DC,酶联免疫反应(ELISA)法检测致敏DC培养液上清IL-12,检测该瘤苗体外诱导特异性CTL的杀伤作用。